WRKY类转录因子调控植物生长发育的麦耐多个方面，
这两个蛋白均定位于细胞核，逆性研究人员通过EST分析鉴定了小麦的录因43个TaWRKY基因，
中科院遗传发育所鉴定出调控小麦耐逆性的中科Y转WRKY转录因子
2012-01-12 16:00 · KayterWRKY类转录因子调控植物生长发育的多个方面，并研究了相关基因的院遗育耐逆性。
中科院遗传与发育生物学研究所陈受宜、传发出调管网清洗随着基因组的鉴定完成，两个基因对植物在不同胁迫下的麦耐耐受性提高可能是由于调控了不同的下游基因。可特异结合典型的逆性顺式作用元件W-box。张劲松和张正斌3个实验室合作分析了小麦中WRKY转录因子基因家族的录因成员，但这类转录因子在植物尤其是中科Y转农作物耐受非生物胁迫方面的作用研究较少。将有更多的小麦WRKY基因得到鉴定。其基因表达也受到多种非生物胁迫的影响。但这类转录因子在植物尤其是农作物耐受非生物胁迫方面的作用研究较少。
Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants
Can-Fang Niu,Wei Wei,Qi-Yun Zhou,Ai-Guo Tian,Yu-Jun Hao,Shou-Yi Chen
WRKY-type transcription factors are involved in multiple aspects of plant growth, development and stress response. WRKY genes have been found to be responsive to abiotic stresses; however, their roles in abiotic stress tolerance are largely unknown especially in crops. Here, we identified stress-responsive WRKY genes from wheat (Triticum aestivum L.) and studied their functions in stress tolerance. Forty-three putative TaWRKY genes were identified and two multiple stress-induced genes TaWRKY2 and TaWRKY19 were further characterized. TaWRKY2 and TaWRKY19 are nuclear proteins, and displayed specific binding to typical cis-element W-box. Transgenic Arabidopsis plants overexpressing TaWRKY2 exhibited salt and drought tolerance compared to controls. Overexpression of TaWRKY19 conferred tolerance to salt, drought and freezing stresses in transgenic plants. TaWRKY2 enhanced expressions of STZ and RD29B, and bound to their promoters. TaWRKY19 activated expressions of DREB2A, RD29A, RD29B and Cor6.6, and bound to DREB2A and Cor6.6 promoters. The two TaWRKY proteins may regulate the downstream genes through direct binding to the gene promoter or via indirect mechanism. Manipulation of TaWRKY2 and TaWRKY19 in wheat or other crops should improve their performance under various abiotic stress conditions.
文献链接：https://onlinelibrary.wiley.com/doi/10.1111/j.1365-3040.2012.02480.x/abstract
通过转基因分析和胁迫下的表现及生理指标分析，鉴定了胁迫应答基因，其基因表达也受到多种非生物胁迫的影响。但可能不是通过直接结合其启动子。WRKY19可结合DREB2A和Cor6.6启动子并激活其表达，该研究鉴定了小麦中可提高耐逆性的WRKY基因，在这些鉴定的基因中有多个基因受到多种胁迫的诱导，WRKY2可结合耐逆及胁迫应答基因STZ和RD29B的启动子并激活其表达。而过表达WRKY19可提高转基因植物的耐旱耐盐和耐冷性。